DNA Sequence Variation within vanA , vanB , vanC-1 , and vanC-2/3 Genes of Clinical Enterococcus Isolates
نویسندگان
چکیده
منابع مشابه
Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci.
Vancomycin-resistant enterococci (VRE) are increasingly isolated from clinical specimens. One hundred clinical isolates of enterococci (E. casseliflavus/E. flavescens [n = 10], E. faecalis [n = 34], E. faecium [n = 43], E. avium [n = 1], E. gallinarum [n = 11], and E. raffinosus [n = 1]) were examined for the presence of vanA, vanB, vanC-1, and vanC-2/3 genes by a single multiplex PCR performed...
متن کاملDetection and differentiation of vanC-1, vanC-2, and vanC-3 glycopeptide resistance genes in enterococci.
The VanC phenotype, as found in Enterococcus gallinarum, E. casseliflavus, and E. flavescens, is characterized by intrinsic low-level resistance to vancomycin. The nucleotide sequences of the vanC-1 gene in E. gallinarum, the vanC-2 gene in E. casseliflavus, and the vanC-3 gene in E. flavescens have been reported, although there is some disagreement as to whether E. flavescens is a legitimate e...
متن کاملvanC cluster of vancomycin-resistant Enterococcus gallinarum BM4174.
Glycopeptide-resistant enterococci of the VanC type synthesize UDP-muramyl-pentapeptide[D-Ser] for cell wall assembly and prevent synthesis of peptidoglycan precursors ending in D-Ala. The vanC cluster of Enterococcus gallinarum BM4174 consists of five genes: vanC-1, vanXY(C), vanT, vanR(C), and vanS(C). Three genes are sufficient for resistance: vanC-1 encodes a ligase that synthesizes the dip...
متن کاملتشخیص ژن‌های vanA, vanB, vanC با روش Multiplex-PCR در ایزوله‌های انتروکوکوس فکالیس و انتروکوکوس فسیوم در بیماران بستری
Background & Aims : After Vancomycin resistant Enterococci (VRE) was first detected in the mid 1980s, VRE spread rapidly worldwide and became one of the most serious problems in many hospitals specially in hospitalized patients. Among different enterococcal species Enterococcus feacalis and Enterococus faecium are the leading causes of life threatening infections. VanA/B/C genes are respons...
متن کاملLow-level vancomycin resistance in Clostridium innocuum.
Low-level vancomycin resistance was observed for 28 clinical Clostridium innocuum isolates and C. innocuum NCIB 10674, whereas teicoplanin was active. DNA from three clinical isolates and the type strain could not be amplified by PCR with primers specific for the genes vanA, vanB, and vanC, suggesting that C. innocuum is intrinsically resistant to vancomycin.
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1998
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.42.1.202